CRISPR/Cas9 GENOM EDITING WORKSHOP
CRISPR/Cas9 technology has revolutionized genome editing, allowing a previously unattainable level of genomic targeting, efficiency, and simplicity. The CRISPR/Cas9 system enables site-specific genomic targeting in virtually any organism. This intensive four-day workshop aims to introduce the participants on using CRISPR/Cas9 system for genome editing.
The workshop will cover CRISPR principles and step-by-step hands-on sessions for better understanding of the technique used.The hands on session includes in vitro sgRNA production, cleavage efficiency testing/screening, culture and transfection of HEK293 cells, monitoring the efficiency of genome editing. In addition, there will be presentation about how to use online sgRNA design tools to select candidate sgRNA sequences.